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pcag gal4 dbd gbp2 vector  (Addgene inc)


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    Structured Review

    Addgene inc pcag gal4 dbd gbp2 vector
    Pcag Gal4 Dbd Gbp2 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcag+gal4+dbd+gbp2+vector/pCAG-Gal4DBD-GBP2+(Plasmid+%2349439)/pmc07261253-1318-5-7
    Average 92 stars, based on 4 article reviews
    pcag gal4 dbd gbp2 vector - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Unblending of transcriptional condensates in human repeat expansion disease
    Article Snippet: IDR-mCherry forward primer: taccggtgacagtggatccggagctac RUNX2 IDR-mCherry forward primer: taccggtGGaagagaggccACTAGTGC IDR-mCherry reverse primer: cgcaagcttcttgtacaattcatccatgc In the GAL4-DBD luciferase system, GAL4 DBD-IDR fusion constructs were expressed from a backbone of GAL4-DBD expression vector [GI-GAL4-DBD (Addgene 42500)], from which GI-GAL4 sequence was removed with EcoRI and XbaI digestion. .. GAL4-DBD was PCR amplified from pCAG-GAL4-DBD-GBP2 vector (Addgene 49439) while introducing a short multiple cloning site C-terminal to GAL4-DBD allowing IDR cloning. .. Synthetic DNA fragments for wt and alanine repeat-deletion IDRs of GSX2, HOXD11, TBX1, EOMES, BHLHE41, and MNX1 sequences were flanked by AsiSI and BsiWI sequences for cloning, and purchased from Genewiz.

    Amplification:

    Article Title: Unblending of transcriptional condensates in human repeat expansion disease
    Article Snippet: IDR-mCherry forward primer: taccggtgacagtggatccggagctac RUNX2 IDR-mCherry forward primer: taccggtGGaagagaggccACTAGTGC IDR-mCherry reverse primer: cgcaagcttcttgtacaattcatccatgc In the GAL4-DBD luciferase system, GAL4 DBD-IDR fusion constructs were expressed from a backbone of GAL4-DBD expression vector [GI-GAL4-DBD (Addgene 42500)], from which GI-GAL4 sequence was removed with EcoRI and XbaI digestion. .. GAL4-DBD was PCR amplified from pCAG-GAL4-DBD-GBP2 vector (Addgene 49439) while introducing a short multiple cloning site C-terminal to GAL4-DBD allowing IDR cloning. .. Synthetic DNA fragments for wt and alanine repeat-deletion IDRs of GSX2, HOXD11, TBX1, EOMES, BHLHE41, and MNX1 sequences were flanked by AsiSI and BsiWI sequences for cloning, and purchased from Genewiz.

    Cloning:

    Article Title: Unblending of transcriptional condensates in human repeat expansion disease
    Article Snippet: IDR-mCherry forward primer: taccggtgacagtggatccggagctac RUNX2 IDR-mCherry forward primer: taccggtGGaagagaggccACTAGTGC IDR-mCherry reverse primer: cgcaagcttcttgtacaattcatccatgc In the GAL4-DBD luciferase system, GAL4 DBD-IDR fusion constructs were expressed from a backbone of GAL4-DBD expression vector [GI-GAL4-DBD (Addgene 42500)], from which GI-GAL4 sequence was removed with EcoRI and XbaI digestion. .. GAL4-DBD was PCR amplified from pCAG-GAL4-DBD-GBP2 vector (Addgene 49439) while introducing a short multiple cloning site C-terminal to GAL4-DBD allowing IDR cloning. .. Synthetic DNA fragments for wt and alanine repeat-deletion IDRs of GSX2, HOXD11, TBX1, EOMES, BHLHE41, and MNX1 sequences were flanked by AsiSI and BsiWI sequences for cloning, and purchased from Genewiz.

    Plasmid Preparation:

    Article Title: Unblending of transcriptional condensates in human repeat expansion disease
    Article Snippet: IDR-mCherry forward primer: taccggtgacagtggatccggagctac RUNX2 IDR-mCherry forward primer: taccggtGGaagagaggccACTAGTGC IDR-mCherry reverse primer: cgcaagcttcttgtacaattcatccatgc In the GAL4-DBD luciferase system, GAL4 DBD-IDR fusion constructs were expressed from a backbone of GAL4-DBD expression vector [GI-GAL4-DBD (Addgene 42500)], from which GI-GAL4 sequence was removed with EcoRI and XbaI digestion. .. GAL4-DBD was PCR amplified from pCAG-GAL4-DBD-GBP2 vector (Addgene 49439) while introducing a short multiple cloning site C-terminal to GAL4-DBD allowing IDR cloning. .. Synthetic DNA fragments for wt and alanine repeat-deletion IDRs of GSX2, HOXD11, TBX1, EOMES, BHLHE41, and MNX1 sequences were flanked by AsiSI and BsiWI sequences for cloning, and purchased from Genewiz.



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    Addgene inc pcag gal4 dbd gbp2 vector
    Pcag Gal4 Dbd Gbp2 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcag+gal4+dbd+gbp2+vector/pCAG-Gal4DBD-GBP2+(Plasmid+%2349439)/pmc07261253-1318-5-7
    Average 92 stars, based on 1 article reviews
    pcag gal4 dbd gbp2 vector - by Bioz Stars, 2026-09
    92/100 stars
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